Fig 4

(A–C) ISH for bdnf in control (A), 5-HT-injected (B), and IL4-injected (C) brains. Red rectangles are enlarged to the right of the main panels. Note significant reduction of bdnf expression after 5-HT. (D, Dʹ) IHC for PCNA and S100β in control (D) and BDNF-injected (Dʹ) brains. (E) Quantification of proliferating glial cells after BDNF injection. (F) IHC for BrdU and HuC/D in control and BDNF-injected brains. (G) Quantification of newborn neurons at 14 dpi after BrdU treatment at Day 2 and Day 3 after PBS and BDNF injection. (H) ISH for ntrk2, which is expressed in periventricular neurons but not in NSCs in the vz. (I) ISH for ngfra, which is expressed in vz. (J) IHC for S100β, NFkB-driven GFP, and PCNA in control brains. To the right of the larger panel are single-fluorescence channels. (K) High-magnification of the medial region of panel J without DAPI. (K1–K3) Single fluorescent channels of panel K. (L) IHC for S100β, NFkB-driven GFP, and PCNA in BDNF-injected brains. To the right of the larger panel are single-fluorescence channels. (M) High-magnification of the medial region of panel L without DAPI. (M1–M3) Single-fluorescence channels of panel M. Scale bars equal 100 μM. Data are represented as mean ± SEM. See also S4–S10 Figs. See S7 Data for supporting information. BDNF, brain-derived neurotrophic factor; BrdU, 5-bromo-2'-deoxyuridine; dpi, days post injection; GFP, green fluorescent protein; HuC/D, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3/4; IHC, immunohistochemistry; IL4, interleukin-4; ISH, in situ hybridization; NFkB, nuclear factor 'kappa-light-chain-enhancer' of activated B-cells; NSC, neural stem cell; PCNA, proliferating nuclear cell antigen; pvz, periventricular zone; S100β, S100 calcium-binding protein B; vz, ventricular zone; 5-HT, serotonin.