Figure 4

(A) Tg(mitfa:gdf6a) and non-transgenic sibling control embryos (left), and quantification of dorsal melanocytes per animal in each group (right). Animals were treated with epinephrine prior to imaging at 5 DPF, n = 8 and 7 for control and Tg(mitfa:gdf6a) groups, respectively, from two independent experiments (N = 2). Scale bar = 1 mm. (B) Diagram of miniCoopR rescue experiment. Animals harboring a mitfa(lf) mutation were injected at the single-cell stage with the miniCoopR vector containing a BMP gene. Animals were evaluated at 5 DPF for the presence of melanocytes. If melanocytes were present, that animal was scored as rescued, whereas animals lacking melanocytes were scored as non-rescued. (C) Percentages of rescued and non-rescued animals following injection of a miniCoopR-BMP vector, n = 361, 193 and 152 for control, dnBMPR, and SMAD1-DVD groups, respectively, from four independent experiments (N = 4). Error bars represent mean + /- SEM. P-values were calculated Student’s t-test for panel A and with Fisher’s exact test with Bonferroni’s correction for panel C, *p<0.05, **p<0.01, ***p<0.001.

Figure 4—source data 1.