FIGURE

Fig. 1.

ID

ZDB-FIG-200116-9

Publication

Chagovetz et al., 2019 - Interactions among Ryanodine Receptor isotypes contribute to muscle fiber type development and function

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Fig. 1.

Mutant ryr alleles do not produce protein products. (A) Schematic of a cross-section of the trunk of a 1-2 dpf zebrafish embryo highlighting muscle organization. SC spinal cord, noto notochord. (B-D) Transverse cryosections of 24 hpf embryo trunks illustrating the RNA expression patterns of (B) ryr1a, (C) ryr1b, and (D) ryr3 detected by WISH. (E-H″) Transverse sections through the trunks of 48 hpf wild-type and mutant embryos immunostained with the 34C (anti-RyR) antibody. Brightfield images (E-H), fluorescent images showing 34C staining (green) (E′-H′), and merged brightfield/fluorescent images (E″-H″) of embryos of indicated genotypes. Each ryr mutation is associated with loss of a distinct component of the normal expression pattern of RyR channels in embryonic muscle. Somitic muscle of triple ryr1a;ryr1b;ryr3 mutants lack all RyR protein detected by the 34C antibody.

Expression Data

Genes:	ryr1a ryr1b ryr3
Antibody:	Ab1-ryr
Fish:	TU ryr1b^z43/z43 ryr1b^z43/z43; ryr3^z45/z45 ryr1a^z42/z42; ryr1b^z43/z43; ryr3^z45/z45
Anatomical Terms:	fast muscle cell slow muscle cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ryr1b^z43/z43 ryr1b^z43/z43; ryr3^z45/z45 ryr1a^z42/z42; ryr1b^z43/z43; ryr3^z45/z45
Observed In:	fast muscle cell slow muscle cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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