IMAGE

Fig. 1.

ID

ZDB-IMAGE-200116-9

Genes

Antibodies

Ab1-ryr

Publication

Chagovetz et al., 2019 - Interactions among Ryanodine Receptor isotypes contribute to muscle fiber type development and function

All Figures

Figures for Chagovetz et al., 2019

Image

Figure Caption

Fig. 1.

Mutant ryr alleles do not produce protein products. (A) Schematic of a cross-section of the trunk of a 1-2 dpf zebrafish embryo highlighting muscle organization. SC spinal cord, noto notochord. (B-D) Transverse cryosections of 24 hpf embryo trunks illustrating the RNA expression patterns of (B) ryr1a, (C) ryr1b, and (D) ryr3 detected by WISH. (E-H″) Transverse sections through the trunks of 48 hpf wild-type and mutant embryos immunostained with the 34C (anti-RyR) antibody. Brightfield images (E-H), fluorescent images showing 34C staining (green) (E′-H′), and merged brightfield/fluorescent images (E″-H″) of embryos of indicated genotypes. Each ryr mutation is associated with loss of a distinct component of the normal expression pattern of RyR channels in embryonic muscle. Somitic muscle of triple ryr1a;ryr1b;ryr3 mutants lack all RyR protein detected by the 34C antibody.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.