FIGURE

Fig. 2.

ID

ZDB-FIG-200116-10

Publication

Chagovetz et al., 2019 - Interactions among Ryanodine Receptor isotypes contribute to muscle fiber type development and function

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Fig. 2.

Formation of Shh-dependent muscle in ryr mutants. Muscle cell type patterning in wild-type and mutant zebrafish 24 hpf embryos was assessed by immunohistochemical staining for expression of the Prox1 and Engrailed nuclear proteins. (A-D) Representative images of Prox1 (magenta) and Engrailed (green) staining of somitic muscle in wild-type (A), MZryr1a (B), MZryr3 (C) and MZryr1a;MZryr3 (D) embryos. Slow muscle pioneer cells (MPs) were identified as cells that expressed both Prox1 and Engrailed antigens; medial fast fibers (MFFs) were identified as cells that expressed only the Engrailed antigen; and superficial slow fibers (SSFs) were identified as cells that expressed only the Prox1 antigen. (E) Quantification of Shh-dependent muscle cell types. One-way ANOVA was used to determine statistical relationships with Sidak's multiple comparisons test used to adjust P-values. n.s., not significant; *P<0.01, **P<0.001.

Expression Data

Antibodies:	Ab1-eng Ab5-prox1
Fish:	TU ryr1a^z42/z42 ryr3^z45/z45 ryr1a^z42/z42; ryr3^z45/z45
Anatomical Terms:	fast muscle cell muscle pioneer slow muscle cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ryr1a^z42/z42 ryr3^z45/z45 ryr1a^z42/z42; ryr3^z45/z45
Observed In:	fast muscle cell muscle pioneer slow muscle cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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