ZFIN ID: ZDB-FIG-200115-42
Monestime et al., 2019 - CoRest1 regulates neurogenesis in a stage-dependent manner. Developmental dynamics : an official publication of the American Association of Anatomists   248(10):918-930 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Day 4

Fig. 6

Enhanced neurogenesis in MZrcor1 mutants. Whole mount RNA in situ hybridization of MZrcor1 and related wild‐type embryos at 13 hpf (A,B) and 24 hpf (C,D) using elavl3 probe reveals ectopic expression of elavl3 in the midbrain and tegmentum at 13 hpf and increased expression along the midline from midbrain to tail at 24 hpf (as indicated by bar). Quantification of domain expression (indicated by bar graph). The average width of the elavl3 domain in the MZrcor1 was 47.7 ± 1.5 µm compared to 36.9 ± 2 µm for the wild‐type controls. E. Dorsal views of 26 hpf (F,I), 50 hpf (G,J), and 98 hpf (H,K) of Tg(elavl3:GFP);rcor1 sbu54/sbu54 mutants show increased differentiating neurons at 24 hpf in midbrain and hindbrain. L. Quantification of fluorescence levels of Tg(elavl3:GFP);rcor1 sbu54/sbu54 larva and sibling controls quantified at 26, 50, and 98 hpf (ROI indicated by white bracket). (*P < 0.05) (Scale bar = 20 µm). hpf, hours postfertilization

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg standard conditions Prim-5 neuron IFL
Long-pec neuron IFL
Day 4 neuron IFL
rcor1sbu54/sbu54; knu3Tg standard conditions Prim-5 neuron IFL
Long-pec neuron IFL
Day 4 neuron IFL
elavl3 WT standard conditions 5-9 somites head ISH
Prim-5 midline column ISH
rcor1sbu54/sbu54 standard conditions 5-9 somites head ISH
5-9 somites midbrain neural rod ISH
Prim-5 midline column ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rcor1sbu54/sbu54 standard conditions 5-9 somites head elavl3 expression mislocalised, abnormal
5-9 somites midbrain neural rod elavl3 expression mislocalised, abnormal
Prim-5 midline column elavl3 expression increased distribution, abnormal
rcor1sbu54/sbu54; knu3Tg standard conditions Prim-5 neuron differentiation increased process quality, abnormal
Long-pec neuron differentiation increased process quality, abnormal
Day 4 neuron differentiation increased process quality, abnormal
Acknowledgments:
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