Characteristic features of dual-initiation and single initiation promoter genes.a Stack bar of proportion of single/dual-initiation promoter genes among translation-associated gene families as indicated. b Gene ontology (GO) categories of single and dual-initiation promoter genes clustered as indicated in green fields. c Sequence composition around dominant YR-initiation and YC-initiation sites of single/dual-initiation promoters. d, e Presence of polypyrimidine stretches in DI promoters. X-axis indicates the length of uninterrupted pyrimidine stretch with respect to YC-initiation frequency (d) and expression levels of YC-initiation sorted by increasing frequency of uninterrupted polypyrimidine stretches (e). f 5′ UTR length of dual-initiation and single initiation YR genes. g Frequency of CTSS in single/dual-initiation promoter genes. h Tag cluster width of single/dual-initiation promoter genes. Boxplots show the 5th, 25th, 50th, 75th, and 95th percentiles where center line is the median.
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