ZFIN ID: ZDB-FIG-191230-642
Prill et al., 2019 - Myomesin is part of an integrity pathway that responds to sarcomere damage and disease. PLoS One   14:e0224206 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Long-pec

Fig 8 <italic>Myomesin1a</italic> is significantly upregulated at early stages of myogenesis in muscle mutants.

qPCR analysis at 19, 24 and 48 hpf revealed a statistically significant up-regulation of myom1a expression in smyd1btm123a, unc45bsb60 and titin2tg287 mutants relative to wild-type embryos at identical stages (A). At 24 hpf, in situ hybridization demonstrated somite-restricted myom1a expression in wild-type siblings (B), which is increased in smyd1btm123a embryos (C). At 24 hpf, wild-type (D) and smyd1btm123a (E) embryos display similar levels of muscle creatine kinase b (ckmb) expression in their skeletal muscle. Compared to wild-type embryos at 24 hpf (D), unc45bsb60 (H) and titin2tg287 (J) show a significant decrease in ckmb expression. At 48 hpf, smyd1btm123a (G), unc45bsb60 (I) and titin2tg287 (K) embryos demonstrated increased ckmb expression in their skeletal muscle when compared to wild-type siblings (E). (Error bars are standard deviation. Expression is normalized using ef1a and wild-type at corresponding stages).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ckmb WT standard conditions Prim-5 skeletal muscle ISH
Long-pec skeletal muscle ISH
heltg287/tg287 standard conditions Prim-5 skeletal muscle ISH
Long-pec skeletal muscle ISH
smyd1btm123a/tm123a standard conditions Prim-5 skeletal muscle ISH
Long-pec skeletal muscle ISH
unc45bsb60/sb60 standard conditions Prim-5 skeletal muscle ISH
Long-pec skeletal muscle ISH
myom1a WT standard conditions 20-25 somites whole organism RTPCR
Prim-5 somite ISH
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
heltg287/tg287 standard conditions 20-25 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
smyd1btm123a/tm123a standard conditions 20-25 somites whole organism RTPCR
Prim-5 somite ISH
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
unc45bsb60/sb60 standard conditions 20-25 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
heltg287/tg287 standard conditions 20-25 somites whole organism myom1a expression increased amount, abnormal
Prim-5 skeletal muscle ckmb expression decreased amount, abnormal
Prim-5 whole organism myom1a expression increased amount, abnormal
Long-pec skeletal muscle ckmb expression increased amount, abnormal
Long-pec whole organism myom1a expression increased amount, abnormal
smyd1btm123a/tm123a standard conditions 20-25 somites whole organism myom1a expression increased amount, abnormal
Prim-5 somite myom1a expression increased amount, abnormal
Prim-5 whole organism myom1a expression increased amount, abnormal
Long-pec skeletal muscle ckmb expression increased amount, abnormal
Long-pec whole organism myom1a expression increased amount, abnormal
unc45bsb60/sb60 standard conditions 20-25 somites whole organism myom1a expression increased amount, abnormal
Prim-5 skeletal muscle ckmb expression decreased amount, abnormal
Prim-5 whole organism myom1a expression increased amount, abnormal
Long-pec skeletal muscle ckmb expression increased amount, abnormal
Long-pec whole organism myom1a expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One