Myomesin1a is significantly upregulated at early stages of myogenesis in muscle mutants.
qPCR analysis at 19, 24 and 48 hpf revealed a statistically significant up-regulation of myom1a expression in smyd1btm123a, unc45bsb60 and titin2tg287 mutants relative to wild-type embryos at identical stages (A). At 24 hpf, in situ hybridization demonstrated somite-restricted myom1a expression in wild-type siblings (B), which is increased in smyd1btm123a embryos (C). At 24 hpf, wild-type (D) and smyd1btm123a (E) embryos display similar levels of muscle creatine kinase b (ckmb) expression in their skeletal muscle. Compared to wild-type embryos at 24 hpf (D), unc45bsb60 (H) and titin2tg287 (J) show a significant decrease in ckmb expression. At 48 hpf, smyd1btm123a (G), unc45bsb60 (I) and titin2tg287 (K) embryos demonstrated increased ckmb expression in their skeletal muscle when compared to wild-type siblings (E). (Error bars are standard deviation. Expression is normalized using ef1a and wild-type at corresponding stages).