ZFIN ID: ZDB-FIG-191230-641
Prill et al., 2019 - Myomesin is part of an integrity pathway that responds to sarcomere damage and disease. PLoS One   14:e0224206 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig 7 Myomesin is absent in damaged skeletal muscle.

Zebrafish embryos treated with galanthamine (GAL) to induce muscle damage by hypercontraction from 28 to 48hpf demonstrated actin and myosin striations in both untreated (A&B) and treated (C&D) wild-type embryos; although GAL treated embryos exhibited fiber disorganization. Myomesin striations are visible in untreated wild-type embryos (E&F) while absent in GAL treated embryo muscle (G&H) even though actin striations, indicative of intact sarcomeres, are present (G’, white arrowheads; H’). qPCR analysis of ckmb and myom1a expression in galanthamine treated and untreated wild-type embryos demonstrated a significant decrease in the expression of both M-band genes at 48hpf (I). (Error bars are standard deviation. Expression is normalized using ef1a and wild-type at 48hpf).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ckmb WT standard conditions Long-pec whole organism RTPCR
WT chemical treatment by environment: galanthamine Long-pec whole organism RTPCR
myom1a WT standard conditions Long-pec whole organism RTPCR
WT chemical treatment by environment: galanthamine Long-pec whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-myom IHC WT control Long-pec skeletal muscle cell
IHC WT chemical treatment by environment: galanthamine Long-pec Not Detected skeletal muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: galanthamine Long-pec skeletal muscle cell ab1-myom labeling absent, abnormal
Long-pec skeletal muscle cell disorganized, abnormal
Long-pec whole organism ckmb expression decreased amount, abnormal
Long-pec whole organism myom1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One