ZFIN Figure: Prill et al., 2019, Fig 7

ZFIN ID: ZDB-FIG-191230-641

ZFIN ID: ZDB-FIG-191230-641

Prill et al., 2019 - Myomesin is part of an integrity pathway that responds to sarcomere damage and disease. PLoS One 14:e0224206 Full text @ PLoS One

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	ckmb myom1a
Antibody:	Ab1-myom
Fish:	WT
Condition:	chemical treatment by environment: galanthamine
Anatomical Terms:	skeletal muscle cell whole organism
Stage:	Long-pec

PHENOTYPE:

Fish:	WT
Condition:	chemical treatment by environment: galanthamine
Observed In:	skeletal muscle cell whole organism
Stage:	Long-pec

Fig 7 Myomesin is absent in damaged skeletal muscle.

Zebrafish embryos treated with galanthamine (GAL) to induce muscle damage by hypercontraction from 28 to 48hpf demonstrated actin and myosin striations in both untreated (A&B) and treated (C&D) wild-type embryos; although GAL treated embryos exhibited fiber disorganization. Myomesin striations are visible in untreated wild-type embryos (E&F) while absent in GAL treated embryo muscle (G&H) even though actin striations, indicative of intact sarcomeres, are present (G’, white arrowheads; H’). qPCR analysis of ckmb and myom1a expression in galanthamine treated and untreated wild-type embryos demonstrated a significant decrease in the expression of both M-band genes at 48hpf (I). (Error bars are standard deviation. Expression is normalized using ef1a and wild-type at 48hpf).

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
ckmb	WT	standard conditions	Long-pec	whole organism	RTPCR
	WT	chemical treatment by environment: galanthamine	Long-pec	whole organism	RTPCR
myom1a	WT	standard conditions	Long-pec	whole organism	RTPCR
	WT	chemical treatment by environment: galanthamine	Long-pec	whole organism	RTPCR

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Qualifier	Anatomy
Ab1-myom	IHC	WT	control	Long-pec		skeletal muscle cell
	IHC	WT	chemical treatment by environment: galanthamine	Long-pec	Not Detected	skeletal muscle cell

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT	chemical treatment by environment: galanthamine	Long-pec	skeletal muscle cell ab1-myom labeling absent, abnormal
		Long-pec	skeletal muscle cell disorganized, abnormal
		Long-pec	whole organism ckmb expression decreased amount, abnormal
		Long-pec	whole organism myom1a expression decreased amount, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One