FIGURE

Fig 4

ID

ZDB-FIG-191230-1546

Publication

Sucharov et al., 2019 - Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway

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Fig 4

Selective breeding just for ectopic bone penetrance modifies the behavior of the <italic>mef2ca</italic><sup><italic>b1086</italic></sup> mutant allele.

(A) Low-penetrance mef2ca heterozygous adults were intercrossed and offspring were grown to adulthood. Fin amputation and genotyping identified mef2ca homozygous adults, which were imaged alongside wild-type siblings. Scale bar: 1mm. (B) 6 dpf full-sibling individuals from a single high-penetrance family were stained with Alizarin Red (bone) and Alcian Blue (cartilage), then genotyped and mef2ca heterozygotes and homozygous wild types were scored for presence of all mef2ca-associated phenotypes. (C) 6 dpf Alizarin Red and Alcian Blue stained skeletons were dissected and flat mounted, lateral view. In these individuals, a shortened symplectic (sy) cartilage phenotype is evident in mef2ca heterozygotes from the high-penetrance strain and mef2ca homozygous mutants from the low-penetrance strain. Scale bar: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mef2ca^b1086/b1086 mef2ca^b1086/+
Observed In:	symplectic
Stage:	Day 6

Phenotype Detail

Acknowledgments

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