FIGURE

FIGURE 8

ID

ZDB-FIG-191230-1143

Publication

Huang et al., 2019 - BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 8

Rescue of BMP2-MO and GDNF-MO phenotypes by heat shock induced BMP2 using Tg(Hsp70:bmp2b-GFP) embryos, as well as by injecting BMP2b mRNA or GDNF mRNA. (a,b) Representative image of 66 hpf embryos obtained from control injection(78/84, 92.8%), (c,d) injection with BMP2b-MO alone(115/151, 76.2%) or (e,f) with BMP2b-MO plus heat-shocked at 10 hpf(120/146, 82.2%) or (g,h) with GDNF mRNA(110/153, 71.9%) or (i,j) with BMP2b mRNA (63/76, 82.9%), (k,l) injection with GDNF-MO alone(115/138) or (m,n) with GDNF-MO plus heat-shocked at 10 hpf(105/125, 84%). Arrowheads indicate phox2b expressing in the intestine. Bar, 100 μm.

Expression Data

Gene:	phox2bb
Fish:	fr13Tg fr13Tg + MO3-gdnfa fr13Tg + MO5-bmp2b
Condition:	heat shock
Knockdown Reagents:	MO3-gdnfa MO5-bmp2b
Anatomical Term:	enteric nervous system
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fr13Tg + MO3-gdnfa fr13Tg + MO5-bmp2b
Condition:	heat shock
Knockdown Reagents:	MO3-gdnfa MO5-bmp2b
Observed In:	enteric nervous system
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.