FIGURE

FIGURE 2

ID

ZDB-FIG-191230-1137

Publication

Huang et al., 2019 - BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development

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FIGURE 2

Neurospheres maintain the charactors for self-renewable and multipotent NCCs. (A) Primary neurospheres were formed at day 7-8(A,e). At 24 h, cells grew with adherence (A,a, red arrow head). After replating, tiny clusters can been seen at day 2 (A,b, red arrow head), which then became a dome (A,c, red arrow head) and neurospheres on day five (A,d, red arrow head). (B) Flow cytometry was used to detect the percentage of P75+ or CD49d+. The positive rate of P75+/CD49d+ was 5.09%. (C) The percentage of P75+/Nestin+ cells were 6.4%, which was detected by immunofluorescence. (D) Primary neurospheres were differentiated into neuron (TUJ1+), glial cells (GFAP+), and smooth muscle cells (α-SMA+).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.