FIGURE

Fig. 6

ID

ZDB-FIG-191015-5

Publication

Javed et al., 2019 - Inhibition of amyloid beta toxicity in zebrafish with a chaperone-gold nanoparticle dual strategy

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Fig. 6

Neurotransmitters and reactive oxygen species (ROS) in the brain of Aβ treated larvae. The biomarkers were measured 5 days post Aβ treatment. Cerebrovascular injection of Aβ peptide at 6 fM per larvae did not significantly (p > 0.05) influence the AchE (a) and GLT (b) levels in zebrafish larvae (n = 10). However, increasing the Aβ dose to 600 fM significantly increased (p < 0.05) AchE and GLT levels. βCas AuNPs (3 ng Au per 4.5 ng βCas), injected 2 h post Aβ treatment, significantly (p < 0.005) reduced the AchE and GLT levels on the 5th day post Aβ (600 fM) treatment. βCas micelles (dose equivalent to βCas in 3 ng βCas AuNPs), in comparison, failed to improve (p > 0.05) the biomarker levels. c ROS generation was significantly (p < 0.005) high in Aβ treated (600 fM) larvae. ROS generation was supressed in βCas AuNPs treated larvae and close to control (n = 10). dRepresentative images of zebrafish larvae expressing DCF/ROS fluorescence when treated with H₂O₂, Aβ, Aβ + βCas AuNPs, and Aβ + βCas. e Larvae’s brain sections were stained for synaptophysin. Aβ-treated larvae presented loss of synaptophysin indicating neurodegeneration. Scale bars in all images are 200 µM. Error bars represent the standard deviation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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