ZFIN ID: ZDB-FIG-190816-32
Fogerty et al., 2019 - Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance. Scientific Reports   9:10302
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 30-44 to Adult

Fig. 6

Adult mutants have normal retinal structure, but mir-183/96/182lri78 survival is depressed. (a,b) Survey of retinal anatomy in adult fish at (a) 12 m or (b) 6 m. Markers used here are equivalent to those used for imaging of larval fish, except that we also examined PCNA and Gfap staining to look for evidence of cell proliferation and gliosis, respectively. (c) Quantification of PCNA labeling in double mutants. PCNA + cells were counted across a 10μm thick retinal cross-section at the level of the optic nerve. Proliferating cells in the optic nerve head and in the ciliary marginal zone were excluded. ns = not significant (ANOVA). For all data points, n = 3. (d) Survival curve of fish from incrosses of WT and mir-183/96/182lri78 heterozygous parents. Each of the two cohorts began the study with a population of 51 fry. (e) Histological analysis of a surviving 15wk mir-183/96/182lri78 homozygote, showing normal retinal anatomy. Arrows in (a), (b), and (e) denote PCNA+ nuclei.

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gfap zrf-1 WT standard conditions Adult Muller cell IHC
zrf-1 mir182lri61/lri61 standard conditions Adult Muller cell IHC
zrf-1 mir182lri81/lri81 standard conditions Adult Muller cell IHC
zrf-1 mir183lri70/lri70 standard conditions Adult Muller cell IHC
zrf-1 mir96lri60/lri60 standard conditions Adult Muller cell IHC
zrf-1 mir96lri69/lri69 standard conditions Adult Muller cell IHC
zrf-1 mir96lri78/lri78 standard conditions Adult Muller cell IHC
zrf-1 mir96lri79/lri79 standard conditions Adult Muller cell IHC
pcna Ab2-pcna WT standard conditions Adult retina IHC
Ab2-pcna mir182lri61/lri61 standard conditions Adult retina IHC
Ab2-pcna mir182lri81/lri81 standard conditions Adult retina IHC
Ab2-pcna mir183lri70/lri70 standard conditions Adult retina IHC
Ab2-pcna mir96lri60/lri60 standard conditions Adult retina IHC
Ab2-pcna mir96lri69/lri69 standard conditions Adult retina IHC
Ab2-pcna mir96lri78/lri78 standard conditions Adult retina IHC
Ab2-pcna mir96lri79/lri79 standard conditions Adult retina IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC WT standard conditions Adult retinal cone cell photoreceptor inner segment
IHC mir182lri61/lri61 standard conditions Adult retinal cone cell photoreceptor inner segment
IHC mir182lri81/lri81 standard conditions Adult retinal cone cell photoreceptor inner segment
IHC mir183lri70/lri70 standard conditions Adult retinal cone cell photoreceptor inner segment
IHC mir96lri60/lri60 standard conditions Adult retinal cone cell photoreceptor inner segment
IHC mir96lri69/lri69 standard conditions Adult retinal cone cell photoreceptor inner segment
IHC mir96lri78/lri78 standard conditions Adult retinal cone cell photoreceptor inner segment
IHC mir96lri79/lri79 standard conditions Adult retinal cone cell photoreceptor inner segment
zpr-3 IHC WT standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC mir182lri61/lri61 standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC mir182lri81/lri81 standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC mir183lri70/lri70 standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC mir96lri60/lri60 standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC mir96lri69/lri69 standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC mir96lri78/lri78 standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC mir96lri79/lri79 standard conditions Adult retinal rod cell rod photoreceptor outer segment
Phenotype Details
Fish Conditions Stage Phenotype
mir182lri61/lri61 standard conditions Adult retina morphology, normal
mir182lri81/lri81 standard conditions Adult retina morphology, normal
Adult retina cell population proliferation normal occurrence, normal
mir183lri70/lri70 standard conditions Adult retina morphology, normal
mir96lri60/lri60 standard conditions Adult retina morphology, normal
mir96lri69/lri69 standard conditions Adult retina morphology, normal
Adult retina cell population proliferation normal occurrence, normal
mir96lri78/lri78 standard conditions Days 30-44 - Days 45-89 whole organism semi-viable, abnormal
Adult retina morphology, normal
mir96lri79/lri79 standard conditions Adult retina morphology, normal
Adult retina cell population proliferation normal occurrence, normal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright.