FIGURE

Fig. 3

ID

ZDB-FIG-191211-2

Publication

Fogerty et al., 2019 - Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance

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Fig. 3

Expression and activity of mutant miRNAs. (a–c) Wild-type and mutant miRNAs were detected in adult zebrafish retina by qPCR. Mutant animals were all homozygotes. Asterisks indicate significantly decreased expression, relative to WT. nd = not detected. (d–f) miRNAs were cloned from adult mutant and wildtype genomic DNA and co-transfected with luciferase reporters encoding miRNA binding sites (MBS) for the indicated miRNAs. Decreased reporter signal indicates miRNA activity, relative to the miR-129 control miRNA. RLU = relative light units. **p < 0.01, ***p < 0.001, ****p < 0.0001, ANOVA with Dunnet’s multiple comparisons test. For all data points, n = 3.

Expression Data

Genes:	dre-mir-96 dre-mir-182 dre-mir-183
Fish:	WT Df(Chr4:dre-mir-183,dre-mir-182)lri81/lri81 dre-mir-96^lri60/lri60 dre-mir-96^{lri79a/lri79a} dre-mir-182^lri61/lri61 dre-mir-183^lri70/lri70 Df(Chr4:dre-mir-96,dre-mir-183)lri69/lri69 Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78
Anatomical Term:	retina
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	Df(Chr4:dre-mir-183,dre-mir-182)lri81/lri81 dre-mir-96^lri60/lri60 dre-mir-96^{lri79a/lri79a} dre-mir-182^lri61/lri61 dre-mir-183^lri70/lri70 Df(Chr4:dre-mir-96,dre-mir-183)lri69/lri69 Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78
Observed In:	retina
Stage:	Adult

Phenotype Detail

Acknowledgments

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