Fig. S2

Requirement of the Actomyosin Cytoskeleton for Ooplasm-Yolk Granules Segregation, Related to Figure 2

(A) Fluorescence/bright-field images of oocytes exposed to DMSO (control), Nocodazole (50μg/ml) or Cytochalasin B (Cyto B, 100μg/ml) or injected with CAMypt mRNA (85 pg/embryo) at 100 mpf. Oocytes were also injected with Dextran-Alexa Fluor 647 (cyan) to label the ooplasm, polystyrene beads (red, 2 μm) to track ooplasm flows, and Ras-eGFP (130 pg/embryo) for controlling the CAMypt overexpression experiments. Scale bars, 100 μm. (B) Averaged speed of injected beads as readout of ooplasm flows during the first 100 mpf. Upper plot: DMSO, blue with N = 3 experiments, n = 3 oocytes; Cytochalasin B (Cyto B), red with N = 3 experiments, n = 3 oocytes; Nocodazole, green with N = 4 experiments, n = 4 oocytes. Lower plot: Ras-eGFP, blue with N = 3 experiments, n = 3 oocytes; CAMypt, red with N = 2 experiments, n = 2 oocytes. (C) Normalized and averaged distance of beads to the animal pole of the oocyte during the first 100 mpf. Color coding and N/n as in (B). 0 and 1 on y axis correspond to animal and vegetal poles, respectively. (D) Averaged speed of marginal YGs marked by 1% NileRed during the first 100 mpf. Color coding and N/n as in (B). Error bars, SEM.