Fig. 2

Requirement of the Actomyosin Cortex for Ooplasm-Yolk Granules Segregation and Spatiotemporal Correlation between Ooplasm Flows and Periodic Bulk Actomyosin Waves

(A) Images of oocytes injected with beads (red) to mark ooplasm flows. Intact oocytes (top row) and oocytes with fragmented actomyosin cortex (“cortex-free,” bottom row) confined in square-shaped boxes.

(B and C) Averaged speed (B) and distance from the animal pole (C, normalized to the AV axis) of injected beads in unconfined control (blue, N = 1 experiment, n = 3 oocytes), confined (red; N = 3, n = 5) and confined and cortex-free oocytes (green; N = 3, n = 3).

(D) Images of cortex-free oocytes expressing Utr-GFP to mark F-actin.

(E) Normalized bulk actin intensity of cortex-free oocytes during the first 100 min post fertilization (mpf). N = 1, n = 3.

(F) Images of F-actin in the blastodisc of oocytes.

(G) Normalized cortical (red, left axis, N = 1, n = 3) and bulk (blue, right axis, N = 1, n = 4) actin intensity during the first 100 mpf.

(H) Images of F-actin in oocytes. Blue point in left panel marks the center of the bulk actin polymerization wave. Dashed line outlines the boundary of the bulk actin polymerization wave.

(I) Averaged bulk actin intensity (red, left axis) and flow speed (blue, right axis) during the first 100 mpf. N = 1, n = 3.