Chorioallantoic membrane of the chicken embryo (CAM). a Bright-field image of the CAM vasculature presenting the functional vascular network from capillaries to big vessels imaged at embryo development day 10 (E10). Bar corresponds to 500 μm; b Vascular network of the CAM presented on the fluorescent angiography with FITC-dextran and an intravenous contrast agent. Bar corresponds to 500 μm; c Scanning electron microscopic picture showing the chorionic epithelium (CH) and two large vessels (VE) in the intermediate mesenchyme of a CAM at day 5 of incubation (reproduced from [652]); d A 12-day CAM incubated on day 8 for 4 days with bioptic specimen of ACN/neuroblastoma cell line tumor xenograft, showing numerous blood vessels around the graft (reproduced from [653]). Bar corresponds to 500 μm; e Immuno-histology of a glioma (U87 tumor) implanted on the CAM with tumor cells in red (vimentin staining and vessels in green(SNA isolectin staining), magnification ×4; with permission from [362], copyright (2005) National Academy of Sciences, USA; (f) a pancreatic adenocarcinoma (BxPC3) nodule (blue, Hoechst) inside of the CAM surrounded by blood vessels (green, SNA isolectin staining). bar corresponds to 100 μm; with permission from [363], copyright, Elsevier (License number 4307171269037); g MCF7-derived tumor implanted on CAM induces angiogenic response. Tip cell (red arrow) and incompletely attached pericytes (yellow arrow)
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