PUBLICATION

Consensus guidelines for the use and interpretation of angiogenesis assays

Authors
Nowak-Sliwinska, P., Alitalo, K., Allen, E., Anisimov, A., Aplin, A.C., Auerbach, R., Augustin, H.G., Bates, D.O., van Beijnum, J.R., Bender, R.H.F., Bergers, G., Bikfalvi, A., Bischoff, J., Böck, B.C., Brooks, P.C., Bussolino, F., Cakir, B., Carmeliet, P., Castranova, D., Cimpean, A.M., Cleaver, O., Coukos, G., Davis, G.E., De Palma, M., Dimberg, A., Dings, R.P.M., Djonov, V., Dudley, A.C., Dufton, N.P., Fendt, S.M., Ferrara, N., Fruttiger, M., Fukumura, D., Ghesquière, B., Gong, Y., Griffin, R.J., Harris, A.L., Hughes, C.C.W., Hultgren, N.W., Iruela-Arispe, M.L., Irving, M., Jain, R.K., Kalluri, R., Kalucka, J., Kerbel, R.S., Kitajewski, J., Klaassen, I., Kleinmann, H.K., Koolwijk, P., Kuczynski, E., Kwak, B.R., Marien, K., Melero-Martin, J.M., Munn, L.L., Nicosia, R.F., Noel, A., Nurro, J., Olsson, A.K., Petrova, T.V., Pietras, K., Pili, R., Pollard, J.W., Post, M.J., Quax, P.H.A., Rabinovich, G.A., Raica, M., Randi, A.M., Ribatti, D., Ruegg, C., Schlingemann, R.O., Schulte-Merker, S., Smith, L.E.H., Song, J.W., Stacker, S.A., Stalin, J., Stratman, A.N., Van de Velde, M., van Hinsbergh, V.W.M., Vermeulen, P.B., Waltenberger, J., Weinstein, B.M., Xin, H., Yetkin-Arik, B., Yla-Herttuala, S., Yoder, M.C., Griffioen, A.W.
ID
ZDB-PUB-180517-6
Date
2018
Source
Angiogenesis   21(3): 425-532 (Review)
Registered Authors
Castranova, Dan, Schulte-Merker, Stefan, Stratman, Amber, Weinstein, Brant M.
Keywords
Angiogenesis, Aortic ring, Chorioallantoic membrane (CAM), Corneal angiogenesis, Endothelial cell migration, Hindlimb ischemia, Intussusceptive angiogenesis, Microfluidic, Myocardial angiogenesis, Plug assay, Proliferation, Recombinant proteins, Retinal vasculature, Tip cells, Vascular network, Vessel co-option, Zebrafish
MeSH Terms
  • Animals
  • Biological Assay/instrumentation
  • Biological Assay/methods*
  • Guidelines as Topic
  • Humans
  • Mice
  • Neoplasms*/blood supply
  • Neoplasms*/metabolism
  • Neoplasms*/pathology
  • Neovascularization, Pathologic*/metabolism
  • Neovascularization, Pathologic*/pathology
PubMed
29766399 Full text @ Angiogenesis
Abstract
The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes