FIGURE

Fig. 11

ID

ZDB-FIG-190801-14

Publication

Nowak-Sliwinska et al., 2018 - Consensus guidelines for the use and interpretation of angiogenesis assays

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Fig. 11

Representative flow-chart of flow cytometry and cell sorting experiments. Overview of samples collection (tumors, organs bearing metastasis, and blood) from mice and the procedure to obtain a single-cell suspension after tissue dissociation, erythrocytes lysis, and cell labeling with fluorescent or paramagnetic-coupled antibodies. Analysis of blood-circulating cells only requires a red blood cell lysis prior to incubation with antibodies of interest. Intracellular antigens can be detected by adding a cell permeabilization step. Fluorescent labeled cells are analyzed by flow cytometry and sorted by FACS while cells labeled with paramagnetic-coupled antibodies can be sorted by MACS. MACS can be used to pre-enrich cells for subsequent FACS sorting. Cell populations of interest are identified in by dot plots analysis of collected data

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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