Fig. 1

Zebrafish tp63^−/− mutant embryos show de-regulated epidermal program. a Whole-mount embryo immunostaining of p63 in wild-type (WT), heterozygous tp63^+/− and homozygous tp63^−/− embryos at 32 h post fertilization (hpf) showing the absence of p63 expression in tp63^−/− mutants. Left, zoomed lateral view of the tail; middle, lateral view of whole-embryo; right, zoomed lateral view of the yolk. YSL, yolk syncytial layer. b Whole-mount in situ hybridization of the tbx5a gene in WT and tp63^−/−embryos at 36 hpf showing expression in eye, heart and pectoral fin bud, the latter one being reduced in tp63^−/− mutant accordingly with the absence of this anatomical structure. Left, lateral view of whole embryo; middle, zoomed lateral view of the pectoral fin bud; right, dorsal view of whole embryo. For a and b, anterior is to the right. For a and b, scale bars represent 250 µm. c Differential analysis of gene expression between WT and tp63^−/− at 36 hpf from RNA-seq (n = 3 biological replicates per condition). The log₂ base mean WT transcript expression levels versus the log₂ fold-change of expression are plotted. Transcripts showing a statistically significant differential expression (p < 0.05) are highlighted in red (upregulated) or blue (downregulated). d Box plot representing the absolute log₂ fold-change of the up- (n = 973) and down-regulated (n = 1,371) transcripts from (c). Center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range. p value according to the Wilcoxon’s rank sum test is shown. Source data are provided as a Source Data file. e Gene Ontology (GO) Biological Process term enrichment of the genes corresponding to the up- and down-regulated transcripts from (c). f Top-10 enrichment of WT expression patterns of the genes corresponding to the up- and down-regulated transcripts from (c). For e and f, the -log₁₀ of p-value for each term is shown