PUBLICATION

Pioneer and repressive functions of p63 during zebrafish embryonic ectoderm specification

Authors
Santos-Pereira, J.M., Gallardo-Fuentes, L., Neto, A., Acemel, R.D., Tena, J.J.
ID
ZDB-PUB-190713-3
Date
2019
Source
Nature communications   10: 3049 (Journal)
Registered Authors
Neto, Ana, Tena, Juan
Keywords
none
Datasets
GEO:GSE123056, GEO:GSE123059, GEO:GSE123057, GEO:GSE123058
MeSH Terms
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Down-Regulation
  • Ectoderm/embryology*
  • Ectoderm/metabolism
  • Protein Binding/genetics
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental*
  • Embryonic Development/genetics*
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism*
  • Animals
  • CRISPR-Cas Systems/genetics
  • Cell Differentiation/genetics
  • Trans-Activators/genetics
  • Trans-Activators/metabolism*
  • Neural Plate/embryology*
  • Neural Plate/metabolism
  • Animals, Genetically Modified
  • Gene Knockout Techniques
  • Epidermis/embryology
  • Epidermis/metabolism
  • Enhancer Elements, Genetic/genetics
  • SOXB1 Transcription Factors/genetics
  • SOXB1 Transcription Factors/metabolism
  • Chromatin/metabolism
  • Models, Animal
  • Zebrafish/embryology
(all 28)
PubMed
31296872 Full text @ Nat. Commun.
Abstract
The transcription factor p63 is a master regulator of ectoderm development. Although previous studies show that p63 triggers epidermal differentiation in vitro, the roles of p63 in developing embryos remain poorly understood. Here, we use zebrafish embryos to analyze in vivo how p63 regulates gene expression during development. We generate tp63-knock-out mutants that recapitulate human phenotypes and show down-regulated epidermal gene expression. Following p63-binding dynamics, we find two distinct functions clearly separated in space and time. During early development, p63 binds enhancers associated to neural genes, limiting Sox3 binding and reducing neural gene expression. Indeed, we show that p63 and Sox3 are co-expressed in the neural plate border. On the other hand, p63 acts as a pioneer factor by binding non-accessible chromatin at epidermal enhancers, promoting their opening and epidermal gene expression in later developmental stages. Therefore, our results suggest that p63 regulates cell fate decisions during vertebrate ectoderm specification.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping