FIGURE

Figure 4 - figure supplement 2

ID

ZDB-FIG-190723-954

Publication

Hardy et al., 2019 - Detailed analysis of chick optic fissure closure reveals Netrin-1 as an essential mediator of epithelial fusion

Other Figures

All Figure Page

Back to All Figure Page

Figure 4 - figure supplement 2

Analyses of mouse Ntn1 knockout fissures during fusion.

(a) Immunofluorescence staining for mouse Ntn1 in wild-type E12.5 eyes post-fusion showed absence of Ntn1 signal (arrowheads) in the distal (A) and medially fused OFM (B), but presence of Ntn1 (arrowheads) in the proximal (C) and optic disc (D) regions. (b) E11.5 Ntn1^-/- embryos did not show any obvious size or gross structural differences during active fusion stages (n = 4 Ntn1^-/- embryos analysed, total = 8 eyes). Ventral tissue at the optic fissures (OF, arrows) appeared to be normally apposed. (c) Sections from Wild-type and Ntn1^-/- optic fissures immmunostained with anti-laminin antibody (green) and counterstained with DAPI and Phalloidin (red) showed mutant OFMs aligned correctly at E11.5 with no clear structural differences observed between the genotypes. Representative sections from distal, medial, and proximal OFMs are shown. OF, optic fissure; NR, neural retina; RPE, retinal pigmented epithelium.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife