ZFIN ID: ZDB-FIG-190723-760
Li et al., 2019 - Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development. Frontiers in molecular neuroscience   12:126 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Day 5

FIGURE 2

CRISPR/Cas9-mediated tubgcp3 knockout results in zebrafish developmental defects. (A) Sanger sequencing displaying a 5-base pair (bp) deletion in tubgcp3 gene in the zebrafish mutants. The black box indicates the deletion. (B)Predicted structure and amino acid sequence of the wild-type and the mutant alleles of Tubgcp3. The 5-bp deletion in tubgcp3 gene was predicted to generate a 99 amino acid (aa)-truncated Tubgcp3 protein without the GRIP1 domain (green box) and GRIP2 domain (purple box). (C) Western blotting analysis showing Tubgcp3 protein decreased in tubgcp3 mutant embryos at 5 dpf. (D) Whole-mount lateral views of the tubgcp3 mutant and sibling embryos at the indicated developmental stages. (E) Higher magnification of the lateral and dorsal views of zebrafish heads from siblings and tubgcp3mutants at 3 and 5 dpf. The brain size is measured based on the fluorescent area in the head of Tg(HuC:GFP). Red dotted lines indicate the brain area of the embryos used for analysis. (F,G) Scatter plot of eye and head size from wild-type siblings and tubgcp3 mutants at 3 and 5 dpf. Data are from 29 embryos for each group. Student’s t-test: ∗∗P < 0.01. Scale bars: 1 mm (D); 200 μm (E).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg standard conditions Protruding-mouth head IFL
Day 5 head IFL
tubgcp3zf3126/zf3126; knu3Tg standard conditions Protruding-mouth head IFL
Day 5 head IFL
tubgcp3 WT standard conditions Day 5 whole organism WB
tubgcp3zf3126/zf3126 standard conditions Day 5 whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tubgcp3zf3126/zf3126 standard conditions Prim-5 brain size, normal
Prim-5 eye size, normal
Long-pec brain size, normal
Long-pec eye size, normal
Protruding-mouth axis decreased length, abnormal
Protruding-mouth brain decreased size, abnormal
Protruding-mouth caudal fin curved, abnormal
Protruding-mouth eye decreased size, abnormal
Protruding-mouth swim bladder uninflated, abnormal
Day 5 axis decreased length, abnormal
Day 5 brain decreased size, abnormal
Day 5 caudal fin curved, abnormal
Day 5 eye decreased size, abnormal
Day 5 swim bladder uninflated, abnormal
Day 5 whole organism tubgcp3 expression decreased amount, abnormal
tubgcp3zf3126/zf3126; knu3Tg standard conditions Protruding-mouth head EGFP expression decreased distribution, abnormal
Day 5 head EGFP expression decreased distribution, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.