PUBLICATION

Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development

Authors
Li, G., Jin, D., Zhong, T.P.
ID
ZDB-PUB-190611-5
Date
2019
Source
Frontiers in molecular neuroscience   12: 126 (Journal)
Registered Authors
Jin, Daqing, Zhong, Tao P.
Keywords
cell cycle, ciliary marginal zone, tubgcp3, zebrafish, γ-TuRC, γ-TuSC
MeSH Terms
none
PubMed
31178691 Full text @ Front. Mol. Neurosci.
Abstract
The centrosomal protein γ-tubulin complex protein 3 (Tubgcp3/GCP3) is required for the assembly of γ-tubulin small complexes (γ-TuSCs) and γ-tubulin ring complexes (γ-TuRCs), which play critical roles in mitotic spindle formation during mitosis. However, its function in vertebrate embryonic development is unknown. Here, we generated the zebrafish tubgcp3 mutants using the CRISPR/Cas9 system and found that the tubgcp3 mutants exhibited the small eye phenotype. Tubgcp3 is required for the cell cycle progression of retinal progenitor cells (RPCs), and its depletion caused cell cycle arrest in the mitotic (M) phase. The M-phase arrested RPCs exhibited aberrant monopolar spindles and abnormal distributed centrioles and γ-tubulin. Moreover, these RPCs underwent apoptosis finally. Our study provides the in vivo model for the functional study of Tubgcp3 and sheds light on the roles of centrosomal γ-tubulin complexes in vertebrate development.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes