FIGURE

Figure 2.

ID

ZDB-FIG-190723-629

Publication

Oh et al., 2019 - Zebrafish model of KRAS-initiated pancreatic endocrine tumor

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Figure 2.

Early KRAS^G12D-responsive pancreatic progenitors contribute to endocrine as well as exocrine cells. (A) The pancreas from 5 dpf double transgenic Tg (ela3I-CRE; LSL-KRAS^G12D) larvae was stained with an exocrine specific marker, CPA. CPA staining was observed in the apical cytoplasm as well-developed apical secretory granules (yellow arrows). The GFP signal was detected in both the membrane and cytoplasm as a surrogate marker of KRAS^G12D activation (white arrows). The merged confocal image showed that the pancreatic progenitor cells expressing oncogenic KRAS^G12D expressed CPA in apical secretory granules of exocrine pancreas (red arrows). (B) The pancreas from 5 dpf double transgenic Tg (ela3I-CRE; LSL-KRAS^G12D) larvae was stained with an endocrine specific marker, insulin. Insulin staining was shown in the cytoplasm of islet β-cells (yellow arrows). The merged confocal image showed that some of pancreatic progenitor cells expressing oncogenic KRAS^G12D co-expressed the endocrine cell marker, insulin (red arrows). Asterisk (*) indicates the auto fluorescence of the gut. Scale bar: 25 µm.

Expression Data

Gene:	ins
Antibodies:	Ab1-CPA Ab1-ins
Fish:	s932Tg; zf3138Tg
Anatomical Terms:	endocrine pancreas pancreatic B cell exocrine pancreas secretory granule pancreatic B cell cytoplasm
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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