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Fig. 2

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ZDB-FIG-190723-263

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Ortega-Recalde et al., 2019 - Zebrafish preserve global germline DNA methylation while sex-linked rDNA is amplified and demethylated during feminisation

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Fig. 2

Fluorescence microscopy of germline cells and their methylation during gonad transformation. a–f Phenotypic sex in zebrafish can be identified using vasa:EGFP expression: during the ‘juvenile ovary’ stage, expression of EGFP is low but consistent between individuals (a, b). Later, expression of EGFP vastly increases in presumptive females (c, d) relative to presumptive males (e, f). This enables sex phenotyping in early stages of sexual differentiation. Scale bars are 500 µm. g, h Methylation in the vasa:EGFP +ve germline cells of female (g) and male (h) fish from the gonad transformation stage until sexual maturity (35–70 dpf). Non-germline control cells were also tested (black lines and dots). For each sample type and timepoint, n = 3 independent biological replicates were used

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.