FIGURE

Fig. 1

ID

ZDB-FIG-190723-261

Publication

Ortega-Recalde et al., 2019 - Zebrafish preserve global germline DNA methylation while sex-linked rDNA is amplified and demethylated during feminisation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Isolation and quantitation of DNA methylation in the zebrafish germline. a–d Fluorescence microscopy of tg(vasa:EGFP) zebrafish embryos and larvae. 1.5 h post-fertilisation (hpf) (a), 24 hpf (b), 48 hpf (c–d). 1.8× view of EGFP +ve labelled cells is shown inset (dashed lines). Scale bars are 500 µm. Forward scatter height (FSC-H) e Flow cytometry plot of 10 zebrafish larvae at 48 hpf. The red dashed square indicates the EGFP +ve population gated for isolation. Blue dots indicate discrete data points (i.e., cellular events), whereas green, yellow and red colouring indicate increasing data density. f Percentage of methylation in CG context from 1 to 28 days post-fertilisation (dpf) in both vasa:EGFP +ve germline cells (green line) and control cells (black line). For each sample type and timepoint, n = 3 independent biological replicates were used, except for 28 dpf vasa:EGFP +ve, which has n = 5 independent biological replicates

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.