ZFIN Image: Ortega-Recalde et al., 2019, Fig. 1

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Fig. 1

Isolation and quantitation of DNA methylation in the zebrafish germline. a–d Fluorescence microscopy of tg(vasa:EGFP) zebrafish embryos and larvae. 1.5 h post-fertilisation (hpf) (a), 24 hpf (b), 48 hpf (c–d). 1.8× view of EGFP +ve labelled cells is shown inset (dashed lines). Scale bars are 500 µm. Forward scatter height (FSC-H) e Flow cytometry plot of 10 zebrafish larvae at 48 hpf. The red dashed square indicates the EGFP +ve population gated for isolation. Blue dots indicate discrete data points (i.e., cellular events), whereas green, yellow and red colouring indicate increasing data density. f Percentage of methylation in CG context from 1 to 28 days post-fertilisation (dpf) in both vasa:EGFP +ve germline cells (green line) and control cells (black line). For each sample type and timepoint, n = 3 independent biological replicates were used, except for 28 dpf vasa:EGFP +ve, which has n = 5 independent biological replicates

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