FIGURE

Fig. 3.

ID

ZDB-FIG-190723-2560

Publication

Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish

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Fig. 3.

UPHD25 treatment decreases Kim-1 expression level after AKI. (A) Immunofluorescence of Kim-1 expression in 6 dpf uninjured (A), 3 dpi gent-AKI +DMSO (B) and 3 dpi gent-AKI +UPHD25 (C) larvae. Apical localization of Kim-1 was shown by overlaying with nuclear counterstain, DAPI (blue). Histological sectioning poses a challenge of obtaining a perpendicular transversal cut to observe Kim-1 apical localization. (C) Shows an ideal perpendicular transversal section to observe apical expression of Kim-1. PTs are outlined in white and RTECs with Kim-1 expression are marked with arrows. (D) Quantification of Kim-1 was acquired via measuring the area of Kim-1 expression in PTs. Mean_NoInjury=0.22 (N=29) vs Mean_{Gent-AKI+DMSO}=5.92 (N=20) vs Mean_{Gent-AKI+UPHD25}=2.71 (N=22). Data pooled from three biological replicates are shown expressed as mean±s.e.m. One-way ANOVA: *P<0.05, ****P<0.001, ns, not significant. Scale bars: 20 μm.

Expression Data

Gene:	EGFP
Antibody:	Ab2-havcr1
Fish:	nz4Tg
Conditions:	chemical treatment by injection: gentamycin chemical treatment by environment: EC 3.5.1.98 (histone deacetylase) inhibitor, chemical treatment by injection: gentamycin
Anatomical Terms:	pronephric duct pronephric duct epithelial cell regenerating tissue pronephric duct
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz4Tg
Conditions:	chemical treatment by injection: gentamycin chemical treatment by environment: EC 3.5.1.98 (histone deacetylase) inhibitor, chemical treatment by injection: gentamycin
Observed In:	pronephric duct regenerating tissue pronephric duct
Stage:	Day 6

Phenotype Detail

Acknowledgments

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