FIGURE

Fig. 3.

ID
ZDB-FIG-190723-2560
Publication
Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish
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Fig. 3.

UPHD25 treatment decreases Kim-1 expression level after AKI. (A) Immunofluorescence of Kim-1 expression in 6 dpf uninjured (A), 3 dpi gent-AKI +DMSO (B) and 3 dpi gent-AKI +UPHD25 (C) larvae. Apical localization of Kim-1 was shown by overlaying with nuclear counterstain, DAPI (blue). Histological sectioning poses a challenge of obtaining a perpendicular transversal cut to observe Kim-1 apical localization. (C) Shows an ideal perpendicular transversal section to observe apical expression of Kim-1. PTs are outlined in white and RTECs with Kim-1 expression are marked with arrows. (D) Quantification of Kim-1 was acquired via measuring the area of Kim-1 expression in PTs. MeanNoInjury=0.22 (N=29) vs MeanGent-AKI+DMSO=5.92 (N=20) vs MeanGent-AKI+UPHD25=2.71 (N=22). Data pooled from three biological replicates are shown expressed as mean±s.e.m. One-way ANOVA: *P<0.05, ****P<0.001, ns, not significant. Scale bars: 20 μm.
Expression Data
Gene:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 6

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Observed In:
Stage: Day 6

Phenotype Detail
Acknowledgments
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