FIGURE

Fig. 2.

ID

ZDB-FIG-190723-2559

Publication

Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish

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Fig. 2.

UPHD25 treatment increases dedifferentiation during AKI. (A-C) Immunofluorescence co-stain of Vimentin (red; cytosolic) and Pax2a (green) in PT of age-matched 6-dpf no injury (A), 3 dpi gent-AKI+DMSO (B) and 3 dpi gent-AKI+UPHD25 (C) fish. (D) Quantification of Vimentin+ RTECs. Mean_{NoInjury+UPHD25}=0 (N=22) vs Mean_{Gent-AKI+DMSO}=8.00 (N=27) vs Mean_{Gent-AKI+UPHD25}=40.31 (N=29). Pax2a+ cells are marked with asterisks and Vimentin+ RTECs are marked with arrowheads. PT is outlined in white. Data pooled from three biological replicates are shown expressed as mean±s.e.m. One-way ANOVA: ****P<0.001. Scale bars: 20 μm.

Expression Data

Antibodies:	Ab1-vim Ab2-pax2a
Fish:	AB
Conditions:	chemical treatment by injection: gentamycin chemical treatment by environment: EC 3.5.1.98 (histone deacetylase) inhibitor, chemical treatment by injection: gentamycin
Anatomical Terms:	pronephric duct pronephric duct epithelial cell regenerating tissue pronephric duct
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	chemical treatment by injection: gentamycin chemical treatment by environment: EC 3.5.1.98 (histone deacetylase) inhibitor, chemical treatment by injection: gentamycin
Observed In:	pronephric duct pronephric duct cell dedifferentiation regenerating tissue cell dedifferentiation regenerating tissue pronephric duct
Stage:	Day 6

Phenotype Detail

Acknowledgments

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