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Figure 1—figure supplement 1.

ID: ZDB-IMAGE-190723-1936
Source: Figures for Mullapudi et al., 2018

Image

Figure Caption

Figure 1—figure supplement 1. <italic>ins,</italic> and not <italic>insb</italic>, is the predominant paralog expressed in zebrafish pancreatic islets.

(A) Brightfield images of 72 hpf wild-type, ins mutant and insb mutant larvae. (B–C) Confocal plane images of the pancreatic islet in 72 hpf wild-type and insb mutant larvae stained for Insulin (white). (D) Fasting blood glucose levels in three mpf wild-type and ins ± animals; mean ± SEM, n = 11 animals. (E) Body length measurements of 50 dpf wild-type and ins ± animals; mean ± SEM, n = 5–6 animals. (F) mRNA expression time course of ins and insb during zebrafish development from 0 to 120 hpf. (G) 4 ng of control (ctrl) or ins morpholino (MO) was injected in non-transgenic or Tg(ins:insb) (insb OE) embryos at the one cell stage and glucose levels measured at 96 hpf; mean ± SEM, n = 3 replicates. Scale bars: 250 μm (A), 5 μm (C, D). P values from t-tests.

Figure Data

Phenotype details

Acknowledgments

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