FIGURE

Fig. 4

ID

ZDB-FIG-190708-34

Publication

Brastrom et al., 2019 - A High-Throughput Assay for Congenital and Age-Related Eye Diseases in Zebrafish

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Fig. 4

Analysis of candidate genes. Whole mount in situ hybridization on 3 dpf retinal sections for (A) rbm24a; (B) and crim1; (C) Phenotypes of control, rbm24a AUG MO at 1.0–1.2 ng, and rbm24a AUG MO at 2.0–2.5 ng; (D) H and E staining of control, rbm24a AUG MO at 1.0–1.2 ng, and rbm24a AUG MO at 2.0–2.5 ng; (E) Phenotypes of control, crim1 AUG MO at 1.0–1.5 ng, and crim1 AUG MO at 2.5–3.0 ng; Same magnification as (A); (F) H and E staining of control, crim1 AUG MO at 1.0–1.5 ng, and crim1 AUG MO at 2.5–3.0 ng. Same magnification as (B); (G) VIZN analysis of control (n = 19), rbm24a AUG MO at 1.0–1.2 ng (n = 18), and rbm24a AUG MO at 2.0–2.5 ng (n = 10) (Mann–Whitney p-value ** = 0.0068, *** = 0.0003); (H) VIZN analysis of control (n = 39), crim1 AUG MO at 1.0–1.5 ng (n = 31), and crim1 AUG MO at 2.5–3.0 ng (n = 18) (Mann–Whitney p-value **** < 0.0001). Yellow arrow indicates lens staining. Light blue arrow denotes ganglion cell layer staining. Dark blue arrow indicates choroid staining. Black arrows point to microphthalmic eyes. White arrow indicates cardiac edema. Scale bars in (A) and (B): 0.04 mm, and in (D) and (F): 0.05 mm.

Expression Data

Genes:	crim1 rbm24a
Fish:	WT
Anatomical Terms:	lens optic choroid retinal ganglion cell layer
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-crim1 WT + MO6-rbm24a
Knockdown Reagents:	MO1-crim1 MO6-rbm24a
Observed In:	eye optomotor response pericardium visual behavior whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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