PUBLICATION
A High-Throughput Assay for Congenital and Age-Related Eye Diseases in Zebrafish
- Authors
- Brastrom, L.K., Scott, C.A., Dawson, D.V., Slusarski, D.C.
- ID
- ZDB-PUB-190414-1
- Date
- 2019
- Source
- Biomedicines 7(2): (Journal)
- Registered Authors
- Slusarski, Diane C.
- Keywords
- cataracts, crim1, microphthalmia, optomotor response, rbm24a, vision, visual assay, visual impairment disorders, zebrafish
- MeSH Terms
- none
- PubMed
- 30979021 Full text @ Biomedicines
Citation
Brastrom, L.K., Scott, C.A., Dawson, D.V., Slusarski, D.C. (2019) A High-Throughput Assay for Congenital and Age-Related Eye Diseases in Zebrafish. Biomedicines. 7(2).
Abstract
Debilitating visual impairment caused by cataracts or microphthalmia is estimated to affect roughly 20 million people in the United States alone. According to the National Eye Institute, by 2050 that number is expected to more than double to roughly 50 million. The identification of candidate disease-causing alleles for cataracts and microphthalmia has been accelerated with advanced sequencing technologies creating a need for verification of the pathophysiology of these genes. Zebrafish pose many advantages as a high-throughput model for human eye disease. By 5 days post-fertilization, zebrafish have quantifiable behavioral responses to visual stimuli. Their small size, many progeny, and external fertilization allows for rapid screening for vision defects. We have adapted the OptoMotor Response to assay visual impairment in zebrafish models of cataracts and microphthalmia. This research demonstrates an inexpensive, high-throughput method for analyzing candidate genes involved in visual impairment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping