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Fig. 1

ID

ZDB-FIG-190627-46

Publication

Otis et al., 2019 - Dietary cholesterol and apolipoprotein A-I are trafficked in endosomes and lysosomes in the live zebrafish intestine

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Fig. 1

Visualization of TF-cholesterol absorption in live, larval zebrafish indicates fatty acids are necessary for dietary cholesterol absorption. TF-cholesterol provided in a lipid-rich meal (5% chicken egg yolk) is absorbed by enterocytes and accumulates in distinct subcellular punctae (A). Representative images show that dietary TF-cholesterol absorption is not observed when it is fed with a lipid-poor meal (5% chicken egg white) (B). Approximately sixfold more TF-cholesterol fluorescence is observed in enterocytes when it is provided with egg yolk relative to egg white (C) (means ± SE, student’s t-test, *P < 0.05). n = 3, with 6–9 fish per n. Arrows indicate colocalization; L, intestinal lumen; N, nucleus; TF-cholesterol, TopFluor-cholesterol.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Gastrointest. Liver Physiol.