ZFIN Image: Otis et al., 2019, Fig. 1

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Figure Caption

Fig. 1

Visualization of TF-cholesterol absorption in live, larval zebrafish indicates fatty acids are necessary for dietary cholesterol absorption. TF-cholesterol provided in a lipid-rich meal (5% chicken egg yolk) is absorbed by enterocytes and accumulates in distinct subcellular punctae (A). Representative images show that dietary TF-cholesterol absorption is not observed when it is fed with a lipid-poor meal (5% chicken egg white) (B). Approximately sixfold more TF-cholesterol fluorescence is observed in enterocytes when it is provided with egg yolk relative to egg white (C) (means ± SE, student’s t-test, *P < 0.05). n = 3, with 6–9 fish per n. Arrows indicate colocalization; L, intestinal lumen; N, nucleus; TF-cholesterol, TopFluor-cholesterol.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Gastrointest. Liver Physiol.