FIGURE

Fig. 4

ID

ZDB-FIG-190627-10

Publication

Yang et al., 2019 - Leptin induces muscle wasting in kras-driven hepatocellular carcinoma (HCC) model in zebrafish

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Fig. 4

Expression of lepr and characterization of lepr mutant zebrafish. (A) RT-qPCR analyses of relative lepr mRNA expression in the liver and skeletal muscle in WT fish. The value of liver expression is arbitrarily set as 1. (B) RT-qPCR analyses of lepr mRNA expression in skeletal muscles in WT, lepr^+/− and lepr^−/− mutant zebrafish. Relative expression levels were the values with WT muscles set as 1. (C) Gross appearance of 4-month-old WT, heterogeneous and homogenous lepr mutant zebrafish. (D) Gross body weight. (E) H&E staining of muscle sections and quantification of MFCSA. (F) Survival curves after kras^G12V induction in heterogeneous mutant fish, homogenous mutant fish and non-kras^G12V controls. In each group, 10 fish were used to initiate the experiments. *P<0.05. Scale bars: 2.5 mm in C; 10 μm in E.

Expression Data

Gene:	lepr
Fish:	WT lepr^{zf3068/zf3068} lepr^zf3068/+
Anatomical Term:	male organism liver
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lepr^{zf3068/zf3068} lepr^zf3068/+ lepr^zf3068/+; gz32Tg lepr^{zf3068/zf3068}; gz32Tg
Condition:	chemical treatment by environment: doxycycline
Observed In:	male organism male organism liver male organism skeletal muscle
Stage:	Adult

Phenotype Detail

Acknowledgments

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