FIGURE

Fig. 6

ID

ZDB-FIG-190627-12

Publication

Yang et al., 2019 - Leptin induces muscle wasting in kras-driven hepatocellular carcinoma (HCC) model in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Effects on hepatocarcinogenesis and muscle wasting of chemical inhibitors targeting downstream of leptin in the signaling pathway. 4-month-old adult male kras^G12V zebrafish were treated with dox for 4 weeks with or without SRP4623, LY294002 or C4493 treatment, in comparison with similarly dox-induced kras^G12V/lepr^+/− fish. In each group, 20 fish were used to initiate the experiments. (A) Gross appearance and liver morphology (left lateral view). Information of targets of chemical inhibitors is shown. (B) Survival curves. (C) Body weight excluding internal viscera. (D) H&E staining of liver sections (left) and quantification of tumor histology (right). (E) IF staining of PCNA (red), Hnf4a (green) and DAPI (blue). Quantification of percentage of proliferating hepatocytes is presented on the right. (F) H&E staining of muscle sections. Quantification of MFCSA is shown on the right. *P<0.05. Scale bars: 2.5 mm in A; 10 μm in D-F.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz32Tg lepr^zf3068/+; gz32Tg
Conditions:	chemical treatment by environment: EC 2.7.10.2 (non-specific protein-tyrosine kinase) inhibitor chemical treatment by environment: doxycycline chemical treatment by environment: LY294002
Observed In:	hepatocyte cell population proliferation male organism male organism hepatocellular carcinoma male organism liver male organism skeletal muscle
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.