FIGURE

Fig. 5

ID

ZDB-FIG-190627-11

Publication

Yang et al., 2019 - Leptin induces muscle wasting in kras-driven hepatocellular carcinoma (HCC) model in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

kras^G12V induction in lepr heterogeneous mutant fish under different levels of feeding. lepr^+/− fish with and without kras^G12V were fed with 25%, 100% and 200% of artemia. Non-mutant WT and kras^G12V fish were fed with 100% of artemia as controls for 4 weeks. In each feeding group, 20 fish were used to initiate the experiments. (A) Gross appearance and liver morphology based on liver-specific GFP expression in kras^G12V fish. (B) Survival curves. (C) Body weight excluding internal viscera. (D) H&E staining of liver sections (left) and quantification (right). (E) IF staining of PCNA (red), Hnf4a (green) and DAPI (blue). Quantification of percentages of proliferating hepatocytes is presented on the right. (F) H&E staining of muscle sections (left) and quantification of MFCSA (right). *P<0.05. Scale bars: 2.5 mm in A; 10 μm in D-F.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz32Tg lepr^zf3068/+; gz32Tg
Conditions:	chemical treatment by environment: doxycycline chemical treatment by environment: doxycycline, increased food availability chemical treatment by environment: doxycycline, limited food availability
Observed In:	hepatocyte cell population proliferation male organism male organism hepatocellular carcinoma male organism liver male organism skeletal muscle
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.