FIGURE

Fig. 1

ID
ZDB-FIG-190613-4
Publication
Bise et al., 2019 - Ciliary neurotrophic factor stimulates cardioprotection and the proliferative activity in the adult zebrafish heart
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Fig. 1

Transcriptional changes after thoracotomy suggest the activation of cardioprotective and EMT genes in the epicardium. a Experimental design. High throughput sequencing (HTPS) of ventricles collected at 0 (control), 1 and 7 days post-thoracotomy (dpt). For each group, RNA was extracted from a pool of 8 ventricles. The encircled numbers indicate differentially expressed genes at each time point. The middle number depicts differentially expressed genes common for both time points. b Heat-map representation of the common 53 differentially expressed genes at 1 and 7 dpt. Genes are grouped according to biological function. Fold changes are represented in log2 scale (blue: log2 < 0; red: log2 > 0). c In-situhybridization of ventricular transversal sections reveals upregulation of several candidate genes (purple) in the epicardial and sub-epicardial region at 7 dpt, compared to control hearts at 0 dpt. The frames indicate the part of the section that is magnified on the right side of each image. n ≥ 3 hearts. Scale bar for the whole section, 100 μm; for the magnified area, 50 μm. d Immunofluorescence staining of ventricular sections of transgenic fish ET27:EGFP(red) with antibodies against cardiac Tropomyosin (TMP, blue) and ColXII (green). At 0 dpt, ET27:EGFP + cells and ColXII are confined to the epicardium. At 7 dpt, ET27:EGFP + cells and ColXII expand and infiltrate the myocardium. Arrows indicate intramyocardial ET27:EGFP + cells. Scale bar, 50 μm. e Quantification of intramyocardial ET27:EGFP + area per ventricular section area. Superficial epicardial ET27 + cells were not included in measurements. n ≥ 3 hearts, 3 sections per heart each. *P < 0.05 with student’s t-test. Error bars represent standard error of the mean (s.e.m.) (This applies to the all subsequent figures)

Expression Data
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Fish:
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Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ NPJ Regen Med