ZFIN ID: ZDB-FIG-190613-4
Bise et al., 2019 - Ciliary neurotrophic factor stimulates cardioprotection and the proliferative activity in the adult zebrafish heart. NPJ Regenerative medicine   4:2 Full text @ NPJ Regen Med
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 1

Transcriptional changes after thoracotomy suggest the activation of cardioprotective and EMT genes in the epicardium. a Experimental design. High throughput sequencing (HTPS) of ventricles collected at 0 (control), 1 and 7 days post-thoracotomy (dpt). For each group, RNA was extracted from a pool of 8 ventricles. The encircled numbers indicate differentially expressed genes at each time point. The middle number depicts differentially expressed genes common for both time points. b Heat-map representation of the common 53 differentially expressed genes at 1 and 7 dpt. Genes are grouped according to biological function. Fold changes are represented in log2 scale (blue: log2 < 0; red: log2 > 0). c In-situhybridization of ventricular transversal sections reveals upregulation of several candidate genes (purple) in the epicardial and sub-epicardial region at 7 dpt, compared to control hearts at 0 dpt. The frames indicate the part of the section that is magnified on the right side of each image. n ≥ 3 hearts. Scale bar for the whole section, 100 μm; for the magnified area, 50 μm. d Immunofluorescence staining of ventricular sections of transgenic fish ET27:EGFP(red) with antibodies against cardiac Tropomyosin (TMP, blue) and ColXII (green). At 0 dpt, ET27:EGFP + cells and ColXII are confined to the epicardium. At 7 dpt, ET27:EGFP + cells and ColXII expand and infiltrate the myocardium. Arrows indicate intramyocardial ET27:EGFP + cells. Scale bar, 50 μm. e Quantification of intramyocardial ET27:EGFP + area per ventricular section area. Superficial epicardial ET27 + cells were not included in measurements. n ≥ 3 hearts, 3 sections per heart each. *P < 0.05 with student’s t-test. Error bars represent standard error of the mean (s.e.m.) (This applies to the all subsequent figures)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
anxa2a AB laceration: pericardial region Adult epicardium ISH
Adult epicardium anatomical region ISH
col12a1b AB laceration: pericardial region Adult epicardium ISH
Adult epicardium anatomical region ISH
EGFP sqet27Et standard conditions Adult epicardium IHC
sqet27Et laceration: pericardial region Adult epicardium IHC
Adult myocardium IHC
mdka AB laceration: pericardial region Adult epicardium ISH
Adult epicardium anatomical region ISH
snai2 AB laceration: pericardial region Adult epicardium ISH
Adult epicardium anatomical region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB laceration: pericardial region Adult epicardium anxa2a expression increased amount, abnormal
Adult epicardium col12a1b expression increased amount, abnormal
Adult epicardium mdka expression increased amount, abnormal
Adult epicardium snai2 expression increased amount, abnormal
Adult epicardium anatomical region anxa2a expression increased amount, abnormal
Adult epicardium anatomical region col12a1b expression increased amount, abnormal
Adult epicardium anatomical region mdka expression increased amount, abnormal
Adult epicardium anatomical region snai2 expression increased amount, abnormal
sqet27Et laceration: pericardial region Adult epicardium EGFP expression increased amount, abnormal
Adult myocardium EGFP expression increased amount, abnormal
Adult myocardium EGFP expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal NPJ Regenerative medicine for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ NPJ Regen Med