ZFIN ID: ZDB-FIG-190613-9
Bise et al., 2019 - Ciliary neurotrophic factor stimulates cardioprotection and the proliferative activity in the adult zebrafish heart. NPJ Regenerative medicine   4:2 Full text @ NPJ Regen Med
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 6

Cntf mutants fail to enhance CM proliferation after thoracotomy. a Schematic drawing of the cntf locus containing 4 exons. UTR (white); translated sequences (orange boxes). The sequence of 154-174 nucleotides (in red) flanking PAM sequence (in blue) in the 3rd exon was targeted by the CRISPR/Cas9-sgRNA RNP complex. b Schematic drawing of the two deletions induced by CRISPR/Cas9. cntfdel207 contains a deletion of 207 bp in the 3rd exon spanning the exon/intron boundary. cntfdel7 comprises a deletion of 7 bp in the middle of the 3rd exon leading to a frameshift and a premature stop codon. c Genotypes and images of wild type and CNTF mutant siblings. F0 mosaic heterozygous candidates were crossed to obtain F1 trans-heterozygous mutants, which are viable without visible phenotype. Scale bar, 5 mm. dExperimental design. e Quantification of PCNA-positive cells among Mef2/DAPI-positive CM nuclei. n ≥ 3 hearts; ≥ 3 sections per heart; *P < 0.05, **P < 0.01. f Quantification of BrdU-positive cells among Mef2/DAPI-positive CM nuclei. n ≥ 3 hearts; ≥ 3 sections per heart; *P < 0.05. gh Transversal heart sections of wt and cntf mutant fish at 7 dpt, immunostained against Mef2 (green, a myocyte nuclear marker) and cell proliferation markers, PCNA (g, red) or BrdU (h, red). All nuclei are labeled with DAPI (blue). Arrows indicate some proliferating CMs. Scale bar for the whole section, 500 μm; for the magnified area, 100 μm; for the zoom of magnified area, 20 μm

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mef2 IHC AB laceration: pericardial region Adult cardiac muscle cell nucleus
IHC cntffri3/+; cntffri4/+ laceration: pericardial region Adult cardiac muscle cell nucleus
Ab2-BrdU IHC AB laceration: pericardial region Adult cardiac muscle cell
IHC Adult heart
IHC cntffri3/+; cntffri4/+ laceration: pericardial region Adult cardiac muscle cell
IHC Adult heart
Ab2-pcna IHC AB laceration: pericardial region Adult cardiac muscle cell
IHC Adult heart
IHC cntffri3/+; cntffri4/+ laceration: pericardial region Adult cardiac muscle cell
IHC Adult heart
Phenotype Details
Fish Conditions Stage Phenotype
AB laceration: pericardial region Adult cardiac muscle cell cell population proliferation increased occurrence, abnormal
Adult heart ab2-brdu labeling increased amount, abnormal
Adult heart ab2-pcna labeling increased amount, abnormal
cntffri3/+; cntffri4/+ laceration: pericardial region Adult cardiac muscle cell cell population proliferation occurrence, ameliorated
Adult heart ab2-brdu labeling amount, ameliorated
Adult heart ab2-pcna labeling amount, ameliorated
Acknowledgments:
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