Fig. 6

Downregulation of STX1B weakened the effects of BBR on anticonvulsant in the PTZ-induced seizure zebrafish model. (A) Hybridization in situ showed that there was a change in the c-fos messenger RNA level in the larval (7 dpf) brain that was induced by BBR in the PTZ plus stx1b morpholino oligos group versus in the three control groups of wild-type, stx1b morpholino oligos injection, and PTZ plus stx1b morpholino oligos (n = 20). (B,C) STX1B downregulation attenuated the efficiency of BBR inhibition on larval overexcited locomotion in terms of speed and distance under non-stimulation conditions and eliminated the action of BBR under dark–light transitions. Swimming trajectories are presented in 2 min recording charts; red tracks indicate over locomotion, while the rectangles indicate the difference between light–dark transitions (n = 24). ^&P < 0.05, ^&&P < 0.01, and ^&&&P < 0.001 vs. PTZ plus stx1b morpholino oligos model; ^𝜃P < 0.05, ^𝜃𝜃P < 0.01, and ^𝜃𝜃𝜃P < 0.001 vs. stx1b morpholino oligos model; ^§P < 0.05 and ^§§§P < 0.001 vs. wild-type in the light condition; ^ΦP < 0.01 and ^ΦΦP < 0.01 indicates light vs. dark.