Fig. 4

Larval seizure-like behavior was reduced by increased STX1B level in a PTZ-induced seizure model. (A) Both stx1b transcription in the wild-type and PTZ models were enhanced in larval (7 dpf) brains by stx1b injection as compared with that following no injection and mock injection (n = 20). (B) Western blotting confirmed differential levels of STX1B protein among the variant groups; notably, the STX1B level was raised in the stx1b-PTZ group as compared with in the PTZ-only group (n = 3). (C) c-fos messenger RNA was decreased by STX1B overexpression in the stx1b-PTZ model versus in the PTZ model group or the mock-PTZ group (n = 20). (D) The larval swimming experiment showed that, with STX1B overexpression, average speed and total distance were not obviously changed but abnormal pathway and overspeed were significantly decreased with 20 min in the dark condition, while photosensitive seizure was inhibited under the condition of light–dark shift in the PTZ-model larvae as compared with in the two groups of the PTZ-only model and the PTZ plus mock injection model (n = 24). The rectangles indicate differential responses between light–dark transitions in the three groups of the PTZ-model larvae. ^∗∗P < 0.01 and ^∗∗∗P < 0.001 vs. wild-type; ^#P < 0.05 and ^##P < 0.01 vs. PTZ model; ^§§ P < 0.01 and ^§§§ P < 0.001 vs. wild-type in the light condition; ^ΦΦΦP < 0.001 indicates light vs. dark.