FIGURE
Fig. 2
- ID
- ZDB-FIG-190115-10
- Publication
- Zhang et al., 2018 - Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function
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Fig. 2
Generation of mtu1 knock-out zebrafish using CRISPR/Cas9 system. (A) Schematic representation of CRISPR/Cas9 target site at exon 4 as used in this study. An allele, mtu1ins32bp was produced by a 32 bp insertion in exon 4 and a truncated 145 amino acid non-functional protein. (B–D) Genotyping of mtu1ins32bp by Sanger sequence, the PAGE RFLP and Western blot analyses. (E) The morphology of mtu1−/−, mtu1+/− and mtu1+/+ zebrafish at 3 dpf. (F) The ratios of genotypes/phenotype of offsprings (F2) in clutches from different F1 mtu1 heterozygous crosses at 10 dpf (n = 350). |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Observed In: | |
| Stage Range: | Protruding-mouth to Adult |
Phenotype Detail
Acknowledgments
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Full text @ Nucleic Acids Res.