FIGURE

Fig. 2

ID

ZDB-FIG-190115-10

Publication

Zhang et al., 2018 - Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function

Other Figures

(all 9)

All Figure Page

Back to All Figure Page

Fig. 2

Generation of mtu1 knock-out zebrafish using CRISPR/Cas9 system. (A) Schematic representation of CRISPR/Cas9 target site at exon 4 as used in this study. An allele, mtu1^ins32bp was produced by a 32 bp insertion in exon 4 and a truncated 145 amino acid non-functional protein. (B?D) Genotyping of mtu1^ins32bp by Sanger sequence, the PAGE RFLP and Western blot analyses. (E) The morphology of mtu1^?/?, mtu1^+/? and mtu1^+/+ zebrafish at 3 dpf. (F) The ratios of genotypes/phenotype of offsprings (F2) in clutches from different F1 mtu1 heterozygous crosses at 10 dpf (n = 350).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	trmu^{zf3047/zf3047}
Observed In:	whole organism
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.