ZFIN ID: ZDB-FIG-181207-18
Chong et al., 2018 - Distinct requirements of E2f4 versus E2f5 activity for multiciliated cell development in the zebrafish embryo. Developmental Biology   443(2):165-172 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4

Effects of combinatorial loss of E2f4 and E2f5 on the MCC transcriptional program in the pronephros and nasal placode. A-D: At 48 hpf, expression of mci (A, B) as well as foxj1b were completely absent in e2f4+/-; e2f5-/- pronephros (C, D). Regions populated with positively labelled cells are flanked by black arrowheads. E, F: At 72 hpf, foxj1a is exclusively expressed on the lateral side of the nasal placodes (E), but was absent in e2f4+/-; e2f5-/- (F) embryos. G, H: At 72 hpf, foxj1b is expressed more widely than foxj1a, found both on the lateral side and within the placode. While the signals on the lateral side (where MCCs predominantly reside, black arrows) were absent in e2f4+/-; e2f5-/- (black arrows), staining of non-MCC cells remained unchanged (white arrowheads). I-L: foxj1a expression was absent at 72 hpf in gmnc embryos (I, J), where similar loss of foxj1b on the lateral side was also observed (K, L). Scale bars = 20 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxj1a e2f4sq5715/+ ; e2f5sq5716/sq5716 standard conditions Protruding-mouth Not Detected peripheral olfactory organ lateral side ISH
e2f5sq5716/+ ; e2f4sq5715/+ standard conditions Protruding-mouth peripheral olfactory organ lateral side ISH
gmncsq34/+ standard conditions Protruding-mouth peripheral olfactory organ lateral side ISH
gmncsq34/sq34 standard conditions Protruding-mouth Not Detected peripheral olfactory organ lateral side ISH
foxj1b e2f4sq5715/+ ; e2f5sq5716/sq5716 standard conditions Long-pec Not Detected pronephros ISH
Protruding-mouth peripheral olfactory organ ISH
Protruding-mouth Not Detected peripheral olfactory organ lateral side ISH
e2f5sq5716/+ ; e2f4sq5715/+ standard conditions Long-pec pronephros ISH
Protruding-mouth peripheral olfactory organ ISH
gmncsq34/+ standard conditions Protruding-mouth peripheral olfactory organ ISH
gmncsq34/sq34 standard conditions Protruding-mouth peripheral olfactory organ ISH
Protruding-mouth Not Detected peripheral olfactory organ lateral side ISH
mcidasl e2f4sq5715/sq5715 standard conditions Long-pec pronephros ISH
e2f5sq5716/sq5716 standard conditions Long-pec Not Detected pronephros ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
e2f4sq5715/+ ; e2f5sq5716/sq5716 standard conditions Long-pec pronephros foxj1b expression absent, abnormal
Protruding-mouth peripheral olfactory organ lateral side foxj1a expression absent, abnormal
Protruding-mouth peripheral olfactory organ lateral side foxj1b expression absent, abnormal
e2f5sq5716/sq5716 standard conditions Long-pec pronephros mcidasl expression absent, abnormal
gmncsq34/sq34 standard conditions Protruding-mouth peripheral olfactory organ lateral side foxj1a expression absent, abnormal
Protruding-mouth peripheral olfactory organ lateral side foxj1b expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 443(2), Chong, Y.L., Zhang, Y., Zhou, F., Roy, S., Distinct requirements of E2f4 versus E2f5 activity for multiciliated cell development in the zebrafish embryo, 165-172, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.