PUBLICATION

Distinct requirements of E2f4 versus E2f5 activity for multiciliated cell development in the zebrafish embryo

Authors
Chong, Y.L., Zhang, Y., Zhou, F., Roy, S.
ID
ZDB-PUB-180916-6
Date
2018
Source
Developmental Biology   443(2): 165-172 (Journal)
Registered Authors
Roy, Sudipto
Keywords
E2f4, E2f5, Gmnc, Multicilin, cilia, multiciliated cell, zebrafish
MeSH Terms
  • Gene Expression Regulation, Developmental/genetics
  • Cell Cycle Proteins/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Animals
  • E2F5 Transcription Factor/metabolism*
  • E2F5 Transcription Factor/physiology
  • Cilia/metabolism
  • Cilia/physiology
  • Cell Differentiation/genetics
  • E2F4 Transcription Factor/metabolism*
  • E2F4 Transcription Factor/physiology
  • Humans
  • Nuclear Proteins/metabolism
  • HEK293 Cells
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
(all 18)
PubMed
30218642 Full text @ Dev. Biol.
Abstract
Multiciliated cells (MCCs) differentiate arrays of motile cilia that beat to drive fluid flow over epithelia. Recent studies have established two Geminin family coiled-coil containing nuclear regulatory proteins, Gmnc and Multicilin (Mci), in the specification and differentiation of the MCCs. Both Gmnc and Mci are devoid of a DNA binding domain: they regulate transcription by associating with E2f family transcription factors, notably E2f4 and E2f5. Here, we have studied the relative contribution of these two E2f factors in MCC development using the zebrafish embryo, which differentiates MCCs within kidney tubules and the nose. We found that while E2f4 is fully dispensable, E2f5 is essential for MCCs to form in the kidney tubules. Moreover, using a variety of double mutant combinations we show that E2f5 has a more prominent role in MCC development in the zebrafish than E2f4. This contrasts with current evidence from the mouse, where E2f4 seems to be more important. Thus, distinct combinatorial activities of the E2f4 and E2f5 proteins regulate the specification and differentiation of MCCs in zebrafish and mice.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
e2f4sq5715/+; e2f5sq5716/sq5716standard conditionsLong-pec
e2f4sq5715/+; e2f5sq5716/sq5716standard conditionsProtruding-mouth
e2f4sq5715/+; e2f5sq5716/sq5716standard conditionsProtruding-mouth
e2f4sq5715/+; e2f5sq5716/sq5716standard conditionsProtruding-mouth
e2f4sq5715/sq5715standard conditionsLong-pec
e2f4sq5715/sq5715; e2f5sq5716/+standard conditionsProtruding-mouth
e2f4sq5715/sq5715; e2f5sq5716/sq5716standard conditionsProtruding-mouth
e2f5sq5716/sq5716standard conditionsLong-pec
e2f5sq5716/sq5716standard conditionsLong-pec
e2f5sq5716/sq5716standard conditionsLong-pec
1 - 10 of 13
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sq34
    		Small Deletion
    sq5715
      		Small Deletion
      sq5716
        		Complex
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        e2f4CRISPR1-e2f4CRISPR
        e2f4CRISPR2-e2f4CRISPR
        e2f5CRISPR1-e2f5CRISPR
        e2f5CRISPR2-e2f5CRISPR
        1 - 4 of 4
        Show
        Fish
        Fish
        e2f4sq5715/sq5715
        e2f4sq5715/sq5715; e2f5sq5716/sq5716
        e2f4sq5715/sq5715; e2f5sq5716/+
        e2f4sq5715/+; e2f5sq5716/sq5716
        e2f4sq5715/+; e2f5sq5716/+
        e2f5sq5716/sq5716
        gmncsq34/sq34
        gmncsq34/+
        WT
        1 - 9 of 9
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        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Chong, Y.L., Zhang, Y., Zhou, F., Roy, S. (2018) Distinct requirements of E2f4 versus E2f5 activity for multiciliated cell development in the zebrafish embryo. Developmental Biology. 443(2):165-172.