FIGURE

Fig. 1

ID

ZDB-FIG-181024-19

Publication

Swaminathan et al., 2018 - Non-canonical mTOR-Independent Role of DEPDC5 in Regulating GABAergic Network Development

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Fig. 1

depdc5 Is Essential for Survival

(A) Whole-mount in situ hybridization showing expression of depdc5 at different stages of development.

(B) Electropherograms showing confirmation of the 4-bp insertion by Sanger sequencing.

(C) Expression of depdc5 transcript in 7-dpf depdc5^+/+, ^+/−, and ^−/− larvae. N = 3; Student’s t test: p(WT versus heterozygote [HT]) = 0.0026; p(WT versus homozygote [HM]) = 0.001.

(D) Survival curve showing that depdc5^−/− embryos have reduced survival, with most larvae dying around 14 dpf (N = 2, n = 15 per genotype).

(E) Images showing that depdc5^−/− larvae do not show gross morphological changes at 3 dpf.

(F) Measurement of body length (top; n = 20/genotype; p = 0.0045 by unpaired Student’s t test) and eye size (bottom; n = 12/genotype; p = 0.7219 by unpaired Student’s t test) showed no significant change in either parameter.

Expression Data

Gene:	depdc5
Fish:	TL depdc5^{udm102/udm102} (TL) depdc5^udm102/+ (TL)
Anatomical Terms:	forebrain hindbrain midbrain notochord whole organism
Stage Range:	14-19 somites to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	depdc5^{udm102/udm102} (TL) depdc5^udm102/+ (TL)
Observed In:	eye whole organism
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

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