FIGURE

Fig. S3

ID

ZDB-FIG-181018-20

Publication

Du et al., 2018 - A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis

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Fig. S3

Example of simultaneous imaging of pre- and postsynaptic sites of retinotectal synapses.

(a) First image of a time-lapse series showing Psd95-DsRedEx labelled postsynaptic sites in the dendritic arbor of a tectal neuron (red) and the Sypb-EGFP labelled presynaptic terminals on the axonal arbor of multiple RGCs (green) in a 4-dpf PGUSG larva with transient expression of elavl3:psd95-DsRedEx. The nasal is upwards. Scale bar, 10 μm. (b) Enlarged view of the boxed region in (a). Scale bar, 5 μm. (c) Enlarged single optical section of the boxed region in (b) showing single-channel images and the composite image. The yellow arrowhead (bottom) indicates the association of a presynaptic terminal labelled by Sypb-EGFP (green, top) with a postsynaptic site labelled by Psd95-DsRedEx (red, middle). Scale bar, 5 μm. (c-i) 2-h time series at 20 min intervals showing the long-term stable overlap of pre- and postsynaptic labels (yellow arrowhead). Single optical section of the same area as in (c) was shown. Time in minutes is indicated in the bottom right corner of each panel.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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