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Fig. 1

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ZDB-FIG-180918-1

Publication

Br�nnimann et al., 2018 - Splitting of circulating red blood cells as an in vivo mechanism of erythrocyte maturation in developing zebrafish, chick and mouse embryos.

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Fig. 1

In vivo proliferation and splitting of circulating red blood cells (RBCs) in zebrafish embryos. (A) Overview of a zebrafish embryo at 48?hours post-fertilization (hpf) in vivo. ISVs, intersegmental vessels; CVP, caudal vein plexus. (B) Phospho-histone H3 (pH3) staining (blue) of the zebrafish caudal vein plexus. In Tg(fli1a:GFP;gata1:DsRed) double transgenic animals, endothelial cells (green) and RBCs (red) are labeled through expression of fluorescent markers as indicated. (C) Example of a pH3-positive circulating RBC. (D) Average extent of pH3-positive cells as percentage of circulating gata1-positive RBCs (n=9; >100 quantified RBCs per animal). (E) Splitting of proliferating RBCs is a four-step process. Following cell division (i), the two somata of a dividing RBC might eventually tangle (ii) at a vascular bifurcation which leads to the subsequent elongation (iii) of the soma-to-soma connection and its eventual rupture (iv). (F) We observed 11.24�0.93 RBC splitting events per hour (n=11; 142 events in total). (G) On average, a splitting event (i.e. phase ii?iv) occurred within 129.56�1.29?s (n=11; 142 events).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Biol.