PUBLICATION
Splitting of circulating red blood cells as an in vivo mechanism of erythrocyte maturation in developing zebrafish, chick and mouse embryos.
- Authors
- Brönnimann, D., Annese, T., Gorr, T.A., Djonov, V.
- ID
- ZDB-PUB-180616-2
- Date
- 2018
- Source
- The Journal of experimental biology 221(Pt 15): (Journal)
- Registered Authors
- Keywords
- Chicken, Circulation, Mouse, Red blood cells, Zebrafish
- MeSH Terms
-
- Erythropoiesis/physiology*
- Chick Embryo/embryology*
- Animals
- Microscopy, Electron
- Zebrafish/embryology*
- Microscopy, Confocal
- Cell Division/physiology
- Erythrocytes/cytology*
- Erythrocytes/ultrastructure
- Mice, Inbred C57BL/embryology*
- PubMed
- 29903841 Full text @ J. Exp. Biol.
Citation
Brönnimann, D., Annese, T., Gorr, T.A., Djonov, V. (2018) Splitting of circulating red blood cells as an in vivo mechanism of erythrocyte maturation in developing zebrafish, chick and mouse embryos.. The Journal of experimental biology. 221(Pt 15).
Abstract
Nucleated circulating red blood cells (RBCs) of developing zebrafish, chick and mouse embryos can actively proliferate. While marrow- or organ-mediated erythropoiesis has been widely studied, transforming in vivo processes of circulating RBCs are under little scrutiny. We employed confocal, stereo- and electron microscopy to document the maturation of intravascular RBCs. In zebrafish embryos (32-72 h post-fertilization), RBC splitting in the caudal vein plexus follows a four-step program: (i) nuclear division with continued cytoplasmic connection between somata; (ii) dumbbell-shaped RBCs tangle at transluminal vascular pillars; (iii) elongation; and (iv) disruption of soma-to-soma connection. Dividing RBCs of chick embryos, however, retain the nucleus in one of their somata. Here, RBC splitting acts to pinch off portions of cytoplasm, organelles and ribosomes. Dumbbell-shaped primitive RBCs re-appeared as circulation constituents in mouse embryos. The splitting of circulating RBCs thus represents a biologically relevant mechanism of RBC division and maturation during early vertebrate ontogeny.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping